Clinical Application of in Vitro Drug Sensitivity for Tumor Cells Using MTT Assay
- VernacularTitle:MTT法测定肿瘤细胞体外药物敏感性的临床应用
- Author:
Xiaowen CHEN
;
Dahua SUN
;
Yunwu LI
- Publication Type:Journal Article
- Keywords:
MTT assay;
chemotherapy;
drug sensitivity test
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To evaluate the in vitro MTT assay for determining the drug sensitivity in fresh human tu- mors. METHODS: We tested the samples from 75 patients with gastric cancer, 55 patients with colorectal carcinoma, 55 patients with bladder cancer and 6 patients with carcinoma of tongue. RESULTS: On the average, the MTT sensitive drug in the above - mentioned first three kinds of solid tumors corresponded with the drug used by clinican experience. The response rate in MTT sensitive group was 73. 8% (48/65) while only 34.0% (17/50) in the control group and 0% (0/10) in MTT resistant group. CONCLUSION: MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer. This test demonstrats that the MTT assay is clinically useful on an individual basis to optimize chemotherapy.
