Effect of 9602 prescription on brain energy metabolism in cerebral ischemia reperfusion mice
- VernacularTitle:9602方对脑缺血再灌小鼠脑能量代谢的影响
- Author:
Yufeng JIANG
;
Yanshu PAN
;
Qifu HUANG
;
Danhui ZHANG
;
Xu JIA
;
Yongbin YAN
;
Xuechun LUO
;
Riqing ZHANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Reperfusion;
Nuclear magnetic resonance;
Energy metabolism;
Mice
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the influence of herbs (9602 prescription) on brain energy metabolism in cerebral ischemia reperfusion mice.METHODS: Ischemia reperfusion in cerebral injury model was duplicated in mice. Using nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC), the brain metabolism were measured. The influence of 9602 prescription on cerebral energy metabolism in ischemia reperfusion mice model was dynamicly observed. RESULTS: Phase Ⅰ: The spectrum of NMR showed that after 10 min of ischemia, the PCr peak dropped significantly, while the Pi peak rose significantly in both the control and the “9602” group. There was no remarkable difference between the two groups. After reperfusion the PCr peak in the control group continued dropping slowly and remained at a low level (55.50?14.94) after 10 min of reperfusion, while after reperfusion the fallen PCr peak in the “9602” group started rising till 76.72?13.37 (P0.05). Phase Ⅱ: The HPLC showed that the cerebral energy charge values of the control group (0.1104?0.0343) were significantly lower than those of the “9602” group (0.2884?0.0552) and the sham-operation group (0.1846?0.0455) (P
