Development of embryonic stem cells into medulloepithelioma in the eyes of nude mice
- VernacularTitle:胚胎干细胞裸鼠眼内诱导形成髓上皮瘤
- Author:
Yongping LI
;
Jian GE
;
Shunong LI
;
Xiufeng ZHONG
;
Jianhua YAN
;
Jianxian LING
;
Guanguang FENG
- Publication Type:Journal Article
- Keywords:
Embryo;
Stem cells;
Mice, nude;
Carcinoma
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the intraocular growth characteristics of mice embryonic stem (ES) cells in nude mice.METHODS: Murine embryonic stem cells (D3 cell lines) were cultured and maintained in an undifferentiated state in vitro, then transplanted into the eyes of nude mice. In 6-45 d, the nude mice were executed for Morphological and immunohistochemical examinations.RESULTS: ES cells were developed into masses which enlarged gradually in the anterior chamber and vitreous cavity. Morphological examination showed different component: cysts, sheets and cords of medullary epithelium and rosettes in the eyes of the nude mice. Most of cells were highly stained by NSE, and some cells were moderately stained by GFAP.CONCLUSION: The embryonic stem cells(D3 cell lines) could differentiated into medulloepithelioma-like tissue in the anterior chamber and vitreous cavity of the Balb/c nude mice.
