Effect of glucagon-like peptide-1(7-36)NH_2 on insulin secretion and membrane potentials of pancreatic islet ?-cells
- VernacularTitle:胰高糖素样肽-1(7-36)NH_2对胰岛?细胞胰岛素分泌和膜电位的作用
- Author:
Yifei WANG
;
Jieyi WANG
;
Guohua WANG
- Publication Type:Journal Article
- Keywords:
Glucagon;
Rats;
Islets of langerhans;
Insulin;
Membrane potential
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the cellular mechanisms by which GLP-1(7-36)NH 2 stimulates insulin secretion in ?-cells. METHODS: Using adherent cell analysis and sorting cytometer (ACAS) by DiBAC 4(3) as a probe,effects of glucagon-like peptide-1(7-36)NH 2 on membrane potential was investigated in cultured neonatal rat pancreatic islet ?-cells,and radio immunological methods were used to determine the amount of insulin secretion in different glucose concentrations. RESULTS: In the presence of 2.8 mmol/L glucose,GLP-1(7-36)NH 2 could not increase insulin secretion and could not change membrane potentials of ?-cells;In the presence of 5.6,16.7 mmol/L glucose,GLP-1(7-36)NH 2 produced a marked increase in insulin secretion and enhanced the depolarization of ?-cells.In 16.7 mmol/L glucose, EGTA or nifedipine completely blocked GLP-1(7-36)NH 2-induced insulin secretion and changes in membrane potentials of ?-cells. CONCLUSION: GLP-1(7-36)NH 2 stimulates insulin secretion in a glucose concentration-dependent manner,it can enhance the depolarization of ?-cells caused by glucose.
