Histopathological observation of cerebral cortex and hippocampus in the mouse with synthetic vascular dementia
- VernacularTitle:拟血管性痴呆小鼠模型皮层及海马细胞病理组织学动态观察
- Author:
Jianxin ZHAO
;
Yuanxiang TIAN
;
Guoming LI
;
Lihui WANG
;
Yanjun ZHEN
- Publication Type:Journal Article
- Keywords:
Dementia, vascular;
Cerebral cortex;
Hippocampus;
Pyramidal cells;
Necrosis
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe pathomorphological changes in cerebral cortex and hippocampus in the mouse with synthetic vascular dementia. METHODS: The synthetic vascular dementia model was produced in the mouse. Animals were killed 7 d, 15 d, and 30 d after the operation, brain tissues were removed and embedded in paraffin. Section of 8?m thickness were stained with hematoxylin-eosin(HE) and Nissl methods, and observed with light microscope. RESULTS: The cerebral cortex in the mouse became thinner on the seventh day, karyopyknosis in partial nervous cells was formed, the number of local neurons was reduced, sieve structure was observed, and glial cells proliferated, with the similar results 15 d and 30 d after operation. Model mouses hippocampal cells in CA 1 area were reduced and almost disappeared 30 d after operation. At the same time, glial cells were abundantly proliferated, tubercles were formed. Cells in CA 2, CA 3 area were also reduced and hippocampal sclerosis occurred. CONCLUSION: Delayed necrosis of hippocampal pyramidal cells may be the pathological basis of ischemia cerebral vascular dementia.
