Role of cellular paracrine in the mechanism of cardiomyocyte hypertrophy induced by stretch
- VernacularTitle:细胞间旁分泌在牵张刺激心肌细胞肥大中的作用
- Author:
Bing FENG
;
Dewen WANG
;
Zuoyu HE
- Publication Type:Journal Article
- Keywords:
Physical stimulation;
Fibroblasts;
Endothelium,vascular;
Cardiomyopathy, hypertrophic
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of cellular paracrine in the mechanism of cardiomyocyte hypertrophy induced by stretch. METHODS: [ 3H]-leu incorporation into cultured cardiomyocytes was measured when stimulated by conditioned-media of myocardial fibroblast and microvascular endothelial cell after stretch. RESULTS: [ 3H]-leu incorporation rate were both elevated significantly stimulated by conditioned-media of myocardial fibroblast and microvascular endothelial cell. And angiotensin II and endothelin of conditioned-media of myocardial fibroblast and microvascular endothelial cell were also elevated significantly. And [ 3H]-leu incorporation rate were inhibited significantly when the specific angiotensin II and endothelin receptor antagonist losartan(1 ?mol/L) and BQ123(1 ?mol/L) were added. [ 3H]-leu incorporation rate were inhibited over 80% when losartan(1 ?mol/L) and BQ123(1 ?mol/L) were added together. CONCLUSION: The activation of myocardial fibroblast and microvascular endothelial cell endocrine stimulated by stretch play a role through paracrine in the pathogenesis of pressure-overload heart hypertrophy.
