Effect of lidocaine on hippocampus neural apoptosis after transient global cerebral ischemia
- VernacularTitle:利多卡因对短暂脑缺血后海马区细胞凋亡的影响
- Author:
Ruiping SONG
;
Xinmin WU
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cerebral ischemia,transient;
Lidocaine
- From:
Chinese Journal of Anesthesiology
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of lidocaine on delayed neuroal death after global cerebral ischemia. Methods Global cerebral ischemia was induced by the occlusions of bilateral commom carotid and vertebral arteries . Twenty-five rabbits were randomly divided into three groups:sham operative group (SH group,n=5) without the four-vessel occlusion; ischemia group(IS group,n=10) with intravenous normal saline of 15 ml followed by the four-vessel occlusion for 5 min; lidocaine group (LI group,n=10) with intravenous lidocaine of 10mg/kg followed by the four-vessel occlusion for 5 min. Three days later, every cerebrum was resected and stained using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and hematoxylin-eosin (HE) methods,to count the amount of the positive stained cells of hippocampus. Results The positive cell amount of HE staining (ischemic change cells) was correlated with that of TUNEL staining (apoptosis cells) (r=0.915).Compared with that in SH group, the amount of apoptosis cells increased significantly in IS and LI groups (P
