Effect of angelica on protein kinase C during myocardial ischemia reperfusion injury in rat
- VernacularTitle:当归对大鼠心肌缺血/再灌注损伤蛋白激酶C的影响
- Author:
Yanlin WANG
;
Chengyao WANG
;
Rui ZENG
- Publication Type:Journal Article
- Keywords:
Angelica sinensis;
Myocardial reperfusion injury;
Cyclic AMP dependent protein kinases
- From:
Chinese Journal of Anesthesiology
1996;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular biological mechanism of the protective effect of angelica on myocardium during ischemia reperfusion Methods The myocardial ischemia/reperfusion was induced with the 40 min cross clamp/120 min declamping of anterior decending coronary artery Forty SD rats were randomly divided into 3 groups: group A without myocardial ischemic reperfusion , group B with intravenous adminisrtration of normal saline 0 8ml/100g before ischemia reperfusion , and group C with intravenous adminisrtration of 25% angelica 0 8ml/100g before ischemia reperfusion The content of protein kinase C (PKC) in cardiac myocyte was measured with immunohistochemical method, and the PKC activity with the isotope lable method Results Compared with those in group B, the myocardial infarct size reduced significantly in group C (P0 05) but increased obviously in group C (P0 05) The PKC activity was significantly higher in group C than that in group A(P
