The role of chlamydia pneumoniae in the course of transformation of C57 BL/6J mouse peritoneal macrophages into foam cells
- VernacularTitle:肺炎衣原体对C57BL/6J小鼠腹膜巨噬细胞泡沫化的影响
- Author:
Junfei JIN
;
Yongzon YANG
- Publication Type:Journal Article
- Keywords:
Chlamydia pneumoniae;
Macrophages;
Foam cells;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to understand the role of chlamydia pneumoniae in the course of macrophages transformation into foam cells experiments with chlamydia pneumoniae standard strain AR-39 wese made. METHOD: C57 BL/6J Mouse peritoneal macrophages C57 BL/6J wese incubated 24 h, and they were divided into 6 groups to be incubated continually: A 1～DMEM; A 2～DMEM+10 6 IFUs/L AR-39; B 1～DMEM+10 mg/L LDL; B 2～DMEM+10 mg/L LDL+10 6 IFUs/L AR-39; C 1～DMEM+10 mg/L OxLDL ;C 2～DMEM+10 mg/L OxLDL+10 6 IFUs/L AR-39. 72 h later, morphological changes of the cells were observed and of intracellular cholesterol of content was detected. RESULTS: 1、Morphological studies: there were no lipid particles in A 1,A 2 and B 1 groups, but the lipid particles could be found in B 2、C 1 and C 2 group, Among six groups, the most lipid particles were seen in C 2 groups. 2、Biochemical detection:The ratio of cholesterol ester to total cholesterol was much higher than 50% in B 2、C 1 and C 2 groups, but was less than 50% in A 1、A 2 and B 1 groups. CONCLUSION: Chlamydia pneumoniae may have played a role in promoting C57 BL/6J mouse peritoneal macrophages into foam cells.
