Regulation of LPS-induced elevation of Ca~(2+) intracellular level of alveolar macrophages in chronic bronchitis by Angelica Sinensis and nifedipine
- VernacularTitle:当归与硝苯吡啶对慢性支气管炎肺泡巨噬细胞胞浆游离钙水平的影响
- Author:
Ze PENG
;
Zhenxiang ZHANG
;
Yongjian XU
;
Zhuola LIU
;
Manjin SONG
- Publication Type:Journal Article
- Keywords:
Angelica Sinensis;
Nifedipine;
Bronchitis;
Macrophages;
Calcium
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore regulation of lipopolysaccharide (LPS)-induced elevation of Ca 2+ intracellular level in alveolar macrophages(AMs) from patients with chronic bronchitis by Angelica Sinensis and nifedipine.METHODS:AMs was obtained from 7 patients with chronic bronchitis and 6 normal controls by bronchoalveolar lavage and intracellular Ca 2+ level was detected after adding Angelica Sinensis, nifedipine or LPS to the supernatant of AMs loaded by Fura-2. RESULTS: In contrast with normal control group (99.65?32.21 nmol/L), intracellular Ca 2+ level in AMs from chronic bronchitis group (189.47?23.69 nmol/L) was increased significantly in the absence of extracellular Ca 2+ but not 1 mmol/L. Intracellular Ca 2+ level in AMs from chronic bronchitis group were significantly increased by adding 10 ?g/mL LPS to the supernatant of AMs. LPS-induced elevation of intracellular Ca 2+ level in AMs from chronic bronchitis group was completely inhibited by Angelica Sinensis or nifedipine.CONCLUSION: Both Anelica Sinensis and nifedipine may inhibit activation of AMs from patients with chronic bronchitis by reducing LPS-induced elevation of intracellular Ca 2+ level in AMs, suggested that these two medicines may inhibit non-specific inflammation of airways in chronic bronchitis.
