Effects of berberine on IL-1 or tumour necrosis factor induced polymorphonuclear leucocyte-endothelium adhesion
- VernacularTitle:小檗碱对IL-1或TNF诱导的多形核白细胞与内皮细胞粘附的影响
- Author:
Yu HAO
;
Quanying QIU
;
Juanjuan WANG
;
Jun WU
- Publication Type:Journal Article
- Keywords:
Endothelium;
Cells;
Cell adhesion;
Drugs, Chinese herbal
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of berberine on IL-1 or tumour necrosis factor (TNF) induced polymorphonuclear leucocyte(PMN)-endothelium adhesion and adhesion molecules.METHODS:Based on the model of human umbilical vein endothelial cell (HUVEC), this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules (CAMs).RESULTS:Berberine inhibited IL-1, TNF-induced HUVEC adhesion for PMN when pretreated HUVEC and antagonised IL-1, TNF-induced upregulation of ICAM-1 on HUVEC. Meanwhile, TNF-stimulated PMN adhesion for HUVEC and CD18 upexpression on PMN was diminished in the presence of berberine.CONCLUSION: Inhibite PMN-endothelium adhesion by downregulating the CAMs expression to inhibite PMN migration across endothelium is one of the mechanisms of antiinflammation of berberine.
