Role and regulation of calcineurin in angiotensin Ⅱ-stimulated cardiac myocyte hypertrophy of rats
- VernacularTitle:钙调神经磷酸酶在血管紧张素Ⅱ刺激的大鼠心肌细胞肥大中的作用及其活性调节
- Author:
Mingui FU
;
Yahong CHEN
;
Shulian LI
;
Song XU
;
Yongzheng PANG
;
Naikui LIU
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Myocardium;
Cell;
Rats;
Phosphatases
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role and regulation of calcineurin(CaN) in angiotensin II(AngⅡ)-stimulated cardiacmyocyte hypertrophy of rats. METHODS: Using AngⅡ to induce the cultured cardiac myocyte hypertrophy of rats, and investigating the effect of CaN inhibitor on [ 3H]-leucine incorporation of AngⅡ-stimulated cardiomyocytes and the regulation of various factors on CaN activity in cardiomyocytes.RESULTS: AngⅡ can stimulate the CaN activity in cultured neonatal rat cardiomyocytes in a dose- and time-dependent manner. In cardiac myocytes incubated with 10, 100, 1000 nmol?L -1 of AngⅡ for 12h, the CaN activities increased respectively by 13%,57%( P
