Effects of EBV infection on growth and apoptosis of nasopharyngeal carcinoma cell line
- VernacularTitle:EB病毒感染对鼻咽癌细胞生长和凋亡的影响
- Author:
Peichun HUANG
;
Hongying LIANG
;
Huihua DENG
;
Minglun ZHAO
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
Nasopharyngeal neoplasms;
Cell line;
Cell division;
Cell death
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased ( P
