Multidrug-resistance antisense inhibits volume-activated chloride current in non-pigmented ciliary epithelial cells
- VernacularTitle:反义多重耐药性基因抑制眼睫状体非色素上皮细胞容积激活性氯电流
- Author:
Lixin CHEN
;
Liwei WANG
- Publication Type:Journal Article
- Keywords:
Ion channels;
Oligonucleotides, antisense;
Drug resistance;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the relationship between multidrug-resistance (MDR1) gene product P-glycoprotein (P-gp) and the volume-activated chloride current. METHODS:The volume-activated chloride current in bovine non-pigmented ciliary epithelial cells was recorded using a whole cell recording technique. An antisense technique was used to inhibit the expression of MDR1 gene. The immunofluorescence of P-gp was monitored with a real-time laser confocal microscope.RESULTS:P-gp immunofluorescence correlated negatively with the concentration of the human MDR1 antisense oligonucleotide. The antisense oligonucleotide inhibited the volume-activated chloride current specifically and partially. The latency of activation of the current increased and the peak current decreased. The percentage of inhibition of peak current correlated positively to the concentration of the antisense oligonucleotide( r =0.99, P
