Expression of bFGF mRNA in pulmonary artery of rat with chronic hypoxia and its effect on tension of rat pulmonary artery in vitro
- VernacularTitle:碱性成纤维细胞生长因子在低氧大鼠肺动脉的表达及其对肺动脉张力的影响
- Author:
Pixin RAN
;
Shengli NIE
;
Yanfang CHEN
;
Shuncu CHEN
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor, basic;
Pulmonary artery;
Rats;
Anoxia
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of basic-fibroblast growth factor (bFGF) in the development of pulmonary hypertension induced by hypoxia. METHODS: 1) The pulmonary arteries of SD rats with hypoxia for one and two weeks were isolated, from which the total RNA were extracted by acid guanidinium thiocyanate-phenol-chlorform .Then the levels of mRNA were measured by RT-PCR. 2) About 3mm-long arterial rings cut from SD rat pulmonary arterial stem were suspended between stainless steelhooks in chamber with warmed (37℃) Kreb's solution. Different concentrations of bFGF were added in a cumulative fashion into the chamber where the rings were suspended. The cumulative concentration response curve was obtained. RESULTS: 1)The levels of bFGF mRNA in pulmonary artery of rats with hypoxia were increased significantly compared with those that without hypoxia (2578?384 counts?min -1 (control) vs 5303?756 (hypoxia) for 1 week and 4054?547 (hypoxia) for 2 weeks, P all
