Oxided low density lipoproteins induce apoptosis with enhanced expression of Fas antigen in vascular endothelial cells
- VernacularTitle:氧化型低密度脂蛋白诱导血管内皮细胞凋亡及Fas抗原表达
- Author:
Ziwang HU
;
Zhihua QUAN
;
Yanfang CHEN
;
Suizhan WANG
;
Shuguang LIN
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Endothelium, vascular;
Cells;
Gene expression
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of oxides of low density lipoprotein (OX - LDL)on apoptosis in vas- cular endothelial cells(EC) and its possible mechanisms. METHODS: Culted vascular ECS were incubated with OX - LDL(0.1?g/L or 0. 3?g/L) for 4 hours, flow cytometer with PI staining methed was used to determine the rate of cellular apoptosis, gel - electrophoresis was performed to observe foe DNA ladder of apoptosis, cellular RNA was isolated and reverse transcription-polymerase chain reaction (RT - PCR)was performed to determine Fas antigen ex- pression. RESULTS:OX - LDL treatment induced apoptosis in EC dose - dependently. No DNA ladder was ob- served in control EC. But it presented in EC beated with low dose of OX - LDL, and was even more when beted with higher dose of OX - LDL. RT - PCR revealed Fas antigen baldly had any expression in basic condition, OX - LDL treatment induced Fas antigen in EC in a dose dependent manner. CONCLUSION: OX - LDL can induce apoptosis in EC, upregulaton of Fas antigen expression maybe one of the possible mechanisms.
