Effects of endothelial nitric oxide synthase gene transfection on cytokines and cAMP production in human phagocytic cells
- VernacularTitle:内皮型一氧化氮合酶基因转染对人吞噬细胞释放细胞因子和生成环核苷酸的影响
- Author:
Liang YAN
;
Qun CAI
- Publication Type:Journal Article
- Keywords:
Phagocytes;
Nitric oxide;
Cytokines;
Adenosine cyclic monophosphate
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHOD: Human endothelial nitric oxide synthase (eNOS) gene was transfected into human phagocytic cell U937 and the effects of gene transfer on cytokines and cAMP production were observed. RESULTS: A functional eNOS was stably expressed in transfected U937 cells, but NO release was undetectable in intact transfectants. However, eNOS gene expression upregulated tumor necrosis factor - a release and downregulated interleukin - 10 and cAMP production in either presence or absence of NOS inhibitor N? - monomethyl - L - arginine. CONCLUSION: The function of tranfected eNOS gene product showed cellular speciality. The effector molecule that changed the produced pattern of cytokines and cAMP in phagocytic cells seems not likely the nitric oxide.
