Protective mechanism of metallothionein on cultivated rat cardiomyocytes in hypoxic preconditioning
- VernacularTitle:金属硫蛋白在培养乳鼠心肌细胞缺氧预处理中的作用
- Author:
Huiying JIN
;
Faqing LI
;
Dexing FANG
;
Suqin LI
;
Weiguo TAN
;
Huabiao CHEN
- Publication Type:Journal Article
- Keywords:
Metallothionein;
Anoxia;
Lipid peroxidation;
(Na~+)-(K~+)-ATPase;
Ca~(2+)- Mg~(2+) ATPase
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Studying the mechanism of protective role of metallothionein (MT) in hypoxic preconditioning(HPC) of cultivated rat cardiomyocytes. METHODS: Using the model of hypoxia/reoxygenation of cultivated rat cardiomyocytes. Determing the contents of MT, malonyldialdehyde (MDA) - metabolism product of lipid peroxidation and the activities of Na+ - K+ ATPase, Ca2+ - Mg2+ ATPase of cardiomyocytes 24 h after HPC, the determining the relevant changes after using MT antibody. RESULTS: After 24 h in HPC, the contents of MT and activities of Na+ - K+ ATPase, Ca2+ - Mg2+ ATPase were obviously higher than those in the control and hypoxia/reoxygenation(P＜ 0. 05 ), and the contents of MDA were decreased remarkedly (P ＜ 0.01 ). Then after using MT antibody, the activities of two enzyme were progressively decreased and the contents of MDA were significanily higher than those in the control and MT antibody - free groups(P ＜ 0.01 ). CONCLUSION: HPC may induce excessive synthesis of MT, and MT can protect myocardial reoxygenation injury by eliminating lipid peroxidation and rising the activities of Na+ - K+ ATPase and Ca2+ - Mg2+ ATPase.
