P53 overexpression in different subcellular location in non small cell lung cancer
- VernacularTitle:非小细胞肺癌中P53表达的不同亚细胞定位及功能意义的研究
- Author:
Qin YANG
- Publication Type:Journal Article
- Keywords:
Carcinoma, non-small-cell lung;
Protein P53;
Patients
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
AIM: Nuclear P53 localization was considered to be proof of mutation, cytoplasmic staining has also been signalled. In this study, special attention has paid to protein subcelluler localization. A different functional meaning may attributed to tumor biological characteristic and evolution. METHODS: Immunohistochemical approach and flow cytometry were used to explored the relationship between P53 overexpression pattern (nuclear and cytoplas- mic) and G1/S checkpoint function of P53 protein in cell cycle. RESULTS: Two patterns of P53 overexpression are related to disease stage significantly. S - phase fraction (SPF) was higher and G0 - G1 phase fraction was lower in P53 nuclear pattern than cytoplasmic (SPF, 42.42? 5 .45/14. 12? 4 .42, G0 - G1: 53. 04 ? 6. 14／７５. 56 ? 4. 46, respectively P
