The effect of homocysteine on airway smooth muscle cells and fibroblasts
- VernacularTitle:同型半胱氨酸对气道平滑肌细胞及成纤维细胞的影响
- Author:
Jianli WANG
;
Mingwu ZHAO
;
Xiaohong WANG
;
Shulian LI
;
Minggui FU
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Cysteine;
Muscle, smooth;
Cells;
Fibroblasts;
Collagen
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of homocysteine (HCY) on proliferation of airway smooth muscle cells and fibroblasts and the effect of HCY on collagen prodution of airway fibroblasts. METHODS: [3H]-TdR incorpora- tion was measured in cultured airway smooth muscle cells. The [3H]-TdR and [3H]-proline incorporation were mea- sured in cultured airway fibroblasts. RESULTS: HCY induced proliferation of airway smooth muscle cells and fibroblasts in a concentration - dependent manner. HCY also induced collagen production of airway fibroblasts in a concentration - dependent manner. The inhibitors of protein kinase C, H7 and polymyxin B, inhibited HCY - induced proliferation of airway smooth muscle cells. CONCLUSIONS: HCY induced proliferation of airway smooth muscle cells and fibroblasts, HCY also induced collagen production of airway fibroblasts. The HCY - induced proliferation of airway smooth muscle cells may be related to the pathway of PKC signal transduction.
