Study of apoptotic thershold of cis-diamminedichloroplatinum and adriamycin on hepatocellular carcinoma
- VernacularTitle:顺铂和阿霉素诱导肝细胞肝癌细胞凋亡阈值研究
- Author:
Xueyun ZHONG
;
Yunxian CHEN
;
Xiaodong SUN
- Publication Type:Journal Article
- Keywords:
Doxorubicin;
Cisplatin;
Hepatoma
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the thershold of hepatocellular carcinoma (HCC) apoptosis induced by adriamycin (ADM) and cis-diamminedichloroplatinum(CDDP). METHODS: Using primary human hepatocellular carcinoma culture, immunofluorescence staining of Hoechst 33 258 in HCC cells ,and flow cytometric assay. RESULTS:24 h after HCC cells were cultured with ADM or CDDP, it were found there were dispersive fluorescences in normal cells nuclei, and compact particulate fluorescences in apoptosis cells nuclei by immunofluorescence staiming of Hoechst 33 258. The rate of HCC cells apoptosis was dependent on doses of ADM and CDDP. HCC cells apoptotic threshold were determined, i.e.ADM was 1.0 ?g/mL and CDDP was 1.5?g/mL (The clinical apoptotic sensitive dosages were ADM 20 mg/m2 and CDDP 30 mg/m2. CONCLUSION: HCC cells apototic threshold of ADM and CDDP were efficient in clinical chemotherapy.
