Role of leukotriene D_4 in promoting proliferation of cultured human airway smooth muscle cells
- VernacularTitle:白三烯D_4在促进培养的人气管平滑肌细胞增殖中的作用
- Author:
Yaling LUO
;
Jinxu WEN
;
Jian XU
;
Jianjun ZHU
- Publication Type:Journal Article
- Keywords:
Muscle,smooth;
Leukotrienes;
Hyperplasia;
Asthma
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether leukotriene D 4(LTD 4) would stimulates proliferation of cultured human airway smooth muscle (ASMC). METHOD: Human ASMC were isolated and subcultured, varying concentration of LTD 4 were added to the media. Cell counts were obtained, [3H]-thymidine([3H]-TdR) incorporation and inositol 1, 4, 5-trisphosphate (IP 3) accumulation were measured. RESULTS: LTD 4(0.1 nmol?L -1～10 nmol?L -1) increased cell number and also increased incorporation of [3H]-TdR and accumulation of IP 3 in a concentration dependent manner(P
