The effect of human complement C_(5b～9) complex on nitric oxide synthesis in glomerular mesangial cells of rats
- VernacularTitle:人补体C_(5b～9)复合物对大鼠肾系膜细胞一氧化氮生成的影响
- Author:
Yingwei WANG
;
Qiuzao HE
;
Huilian QIN
;
Renxian TANG
;
Fengguang GAO
;
Guangyi ZHANG
- Publication Type:Journal Article
- Keywords:
Complement membrane attack complex;
Glomerular mesangium;
Cells;
Nitric oxide;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of human complement C 5b～9 complex on nitric oxide(NO) synthesis of glomerular mesangial cells (MC). METHODS: First, the human complement C 5b～9 complexes were isolated and glomerular MC of rats were cultured. Second, the MC were stimulated with C 5b～9 complex and changes of metabolism products of NO(NO 3 and NO 2) in MC culture supernatant at 6,24 and 48 hours after C 5b～9 stimulating were detected. Moreover, cGMP levels in cultured MC were also measured. RESULTS: NO 3/NO 2 contents from culture supernatant and cGMP levels in MC were increased parallelly after C 5b～9 complex stimulation. Further, NO synthesis was inhibited by L-NG-nitro-arginine-methylester(L-NAME). CONCLUSION: NO synthesis of rat glomerular MC was incerased by human complement C 5b～9 stimulation.
