Mechanism of the inhibitory effect of polymorphonuclear leukocytes on the production of tumor necrosis factor in human phagocytic cells
- VernacularTitle:人中性粒细胞抑制吞噬细胞生成肿瘤坏死因子机制的研究
- Author:
Liang YAN
- Publication Type:Journal Article
- Keywords:
Neutrophils;
Phagocytes;
Azides;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the mechanism of the inhibitory effect of human polymorphonuclear leukocytes (PMN) on the production of tumor necrosis factor-? (TNF-?) in U937 cells. METHODS: PMA differentiated U937 cells were cocultured with PMN in the presence of lipopolysaccharide. Sodium azide was added in the culture. The supernatants of cell culture were collected and TNF-? was assayed with ELISA. RESULTS:The inhibitory effect of PMN on the production of TNF-? in U937 cells was observed. Sodium azide restored the TNF-? production in U937 cells/PMN coculture to the extent of 45% in concentration of about 10～15 ?mol/L. These concentrations of sodium azide did not affect TNF-? production by U937 cells. The restoration effect of sodium azide on PMN inhibition was not related to blocking of myeloperoxidase (MPO) activity. The MPO inhibitors 3-amino-1,2,4,-triazole and 4-aminobenzoyl hydrazide had few effect on PMN inhibition. CONCLUSION: These results indicated that PMN inhibition depended on the cell-cell interactions. Sodium azide may be a useful reagent for further studying this mechanism.
