The effects of rhIL-1? on human fetal islets function and IL-6 production
- VernacularTitle:重组人白细胞介素-1?对人胚胎胰岛分泌白细胞介素-6及对胰岛分泌功能的影响
- Author:
Rui SUN
;
Zhigang TIAN
;
Jianhua ZHANG
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Human;
Embryo;
Islets of langerhans;
Interleukin-6;
Interleukin-1
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study rhIL-1? effects on fetal islet function and IL-6 production in vitro METHODS: Islets from fetal pancreas was separated by collagenase type V (0 5 mg/mL) and cultured in vitro The islets were exposed to culture medium alone for 48 h or with different concentration of rhIL-1? The supernatants of culture of human fetal islets were assayed for IL-6, insulin and glucagon RESULTS:(1) IL-6 activity was increased 4 0 folds (74-294 mU/islet) when islets were exposed to rhIL-1?(20U/mL); (2) IL-6 McAb significantly reduced IL-6 activity in islet supernatants from control group or islet exposed to rhIL-1? treated group; (3)IL-6 mRNA in human fetal islet exposed to rhIL-1? is higher than control in dot hybridization; (4) Soluble insulin and cellular insulin within islet released to supernatants was slightly decreased (0 48～0 78 IU/islet and 0 65～0 79 IU/islet); (5) Glucagon secretion was significantly increased 3 2 folds (1 0～3 2 pg/islet) CONCLUSION: Pancreatic islets produce IL-6 is up-regulated by rhIL-1? On the other hand, Il-6 produced by the islet may act as a costimulator for autoreactive B and T lymphocytes in autoimmune diabetes
