The influence of phosphocreatine on expression of mitochondrial sarcomeric creatine kinase mRNA of myocardium ischemia and reperfusion in rats
- VernacularTitle:磷酸肌酸对大鼠心肌缺氧、复氧心肌线粒体肌酸激酶肌膜型亚基mRNA表达水平的影响
- Author:
Fang LI
;
Hui LI
;
Shijun ZHU
;
Xiaoming WANG
;
Kangzhen YU
- Publication Type:Journal Article
- Keywords:
Rats;
Myocardium;
Anoxia;
RNA, messenger;
Phosphocreatine
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: Rat myocardial mRNA was extracted and mitochondrial sarcomeric creatine kinase (sMiMi-CK) was used as labled probed in vitro myocardial ischemia/reperfusion rats. Northern blot was performed to determine the changes of sMiMi-CK mRNA in different periods of ischemia/reperfusion and the protective effect of phosphocreatine (PCr).RESULTS:During early ischemial (10 min) mRNA was decreased about 18 24%. After reperfusion mRNA decreased again. PCr effectively prevented the decrease of mRNA expression of sMiMi-CK. CONCLUSION:The results indicated that PCr may possess protective effect on anoxic and reoxygenated myocardium.
