Propofol combined with diazepam synergistically potentiates the GABA-activated chloride current in rat sensory neurons
- VernacularTitle:联合使用异丙酚和地西泮对鼠感觉神经元?-氨基丁酸调控的氯电流的影响
- Author:
Ailin LUO
;
Sugiyama KAZUHIDE
;
Yuke TIAN
- Publication Type:Journal Article
- Keywords:
Propofol;
Diazepam;
Drug synergism;
?-aminobutyric acid
- From:
Chinese Journal of Anesthesiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine effects of propofol combined with diazepam on GABA-activated chloride currents(IGABA). Methods Whole-cell patch clamp recordings were made from cultured rat dorsal root ganglionic neurons dissociated by collagenase and trypsin, and trituration. 3 ?mol/L GABA was administered by pressure ejection, meanwhile, propofol and diazepam were dissolved in the external solution and were given with the "Y tube" technique. Results Co-application of propofol(0.3, 1.0, 3.0?mol/L) and diazepam (100 nmol/L) potentiated the IGABA ,which was significantly larger than the sum of that potentiated by either drug alone. Diazepam (100 nmol/L ) shifted the concentration-response curve of the propofol-potentiated IGABA to the left in parallel fasion, the EC50 value of propofol was decreased by diazepam from (7.6 ?1.8) ?mol/L to (3.9?1.1 )?mol/L.Conclusions Propofol combined with diazepam synergistically potentiates GABA-gated chloride currents in rat sensory neurons.
