Effects of pulmonary arterial endothelium cells injuryed by tumor necrosis factor ? on proliferation of pulmonary arterial smooth muscle cells and the interference effects of heat stress response
- VernacularTitle:肿瘤坏死因子-?损伤的肺动脉内皮细胞对肺动脉平滑肌细胞增殖的影响及内皮细胞热应激反应的干扰作用
- Author:
Ling PEI
;
Junke WANG
;
Wen FU
- Publication Type:Journal Article
- Keywords:
Pulmonary Ar-tery;
Endothelium,vascular;
Muscle,smooth,vascular;
Heat-shock response;
Cell cycle
- From:
Chinese Journal of Anesthesiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pulmonary arterial endothelium cells(PAEC) injuryed by tumor necrosis factor ? (TNF-?) on the proliferation of pulmonary arterial smooth muscle cells (PASMC) and the influence of heat stress response (HSR) on it .Methods Normal PAEC or PAEC endured with HSR were incubated with TNF-? at the concentrations of 500, 1000 and 2000u/ml in 1 hour, then cultured in DMEM without serum in 24 hours, the upper liquid was collected to prepare the endothelial cell-conditioned medium liquid (EC-CMⅠ or EC-CMⅡ ), in which PASMC were incubated in 24 hours as group Ⅰ or group Ⅱ respectively. The normal endothelial cell-conditioned medium liquid was also prepared to incubate PASMC in 24 hours as group Ⅲ.The PASMC were incubated without the endothelial cell-conditioned medium liquid as group Ⅳ.Flow cytometry was applied to determining the intracellular DNA content of the incubated PASMC. The fractions of different cytocycle phases were calculated according to the areas under the curves of DNA content.Results Compared with those of group Ⅳ, the percentage of PASMC in G0-G1 phases increased markedly ,and in S and G2-M phases decreased significantly in group Ⅲ (P
