Effect of antioxidant and NF_?B on the induction of iNOS gene in rat pulmonary microvascular endothelial cells in vitro
- VernacularTitle:抗氧化剂和NF_?B对大鼠肺微血管内皮细胞iNOS表达的影响
- Author:
Qinghang LIU
;
Huiming JIN
;
Zhaohui WU
;
Yu CHEN
;
Kai LIU
- Publication Type:Journal Article
- Keywords:
Nitric Oxide;
Antioxidants;
Endothelium
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of nuclear factor ?B (NF ?B) in the induction of iNOS gene by TNF? and LPS in endothelial cells and the effect of antioxidant on the induction of iNOS. METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NF ?B in the cell nucleole was detected with electrophoretic mobility shift assay.RESULTS:(1)NO production and iNOS mRNA expression induced by LPS and TNF? was blocked by pyrrolidine dithiocarbamate(PDTC) or N-acetylcysteine(NAC). (2)LPS and TNF? triggered the activation and translocation of NF ?B, and PDTC or NAC inhibited the activation of NF ?B induced by LPS and TNF?.CONCLUSIONS:(1)The induction of iNOS gene by TNF? and LPS is dependent on the activation of NF ?B.(2)Antioxidants may inhibit the induction of iNOS gene through the inhibition of NF ?B activation.
