Preliminary Observation of Mycoplasma Genitalium under Electron Microscopy and Atomic Force Microscopy
- VernacularTitle:电镜及原子力显微镜观察生殖支原体形态学的初步探讨
- Author:
Dan LUO
;
Ning GU
;
Guojun LIANG
- Publication Type:Journal Article
- Keywords:
Microscopy, atomic force Microscopy, electron
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further identify the isolated Mycoplasma genitalium (Mg) from the high risk population of STD in China. Mothods The morphology of Mg standard strains and clinical isolates were observed under electron microscopy (EM) and atomic force microscopy (AFM). Mg sample preparation for AFM was fixed on mica and AFM images of scanning observation were captured by contacting and tapping modes and operated under normal atmospheric pressure and temperature. Results and Conclusion Mg had a diversity of shapes, including flask, pear, spindle or round shape with projecting neck portion and slightly broadened terminal structure under EM. The morphologic features under AFM were similar to those observed by EM. The typical Mg showed shapes of flask and pear with noticeable narrow ring or mark in the neck portion. The size of Mg measured under EM and AFM was similar as well. Mycoplasma