Effect of Integrin Expression on Procollagen Synthesis of Fibroblasts from Scleroderma
- VernacularTitle:整合素表达对硬皮病成纤维细胞合成原胶原的影响
- Author:
Wei HONG
;
Wanqin LIAO
;
Xiantao KONG
- Publication Type:Journal Article
- Keywords:
Scleroderma, systemic Scleroderma, circumscribed Fibroblasts Integrins Procollagen
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Investigation of the effect of integrin on fibroblasts from scleroderma in the production of procollagen. Methods Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin ? 5 or ? 1 subunit on scleroderma fibroblasts respectively, then the changes of procollagen mRNA were detected by RT PCR. Results The expression of integrin ? 5 or ? 1 subunit could be specifically inhibited by their antisense oligonucleotides respectively. Decreased expression of fibroblast integrin ? 5 or ? 1 subunit significantly lowered mRNA of procollagen ? 1(Ⅰ) and ? 1(Ⅲ). Conclusion Overproduction of procollagen may be inhibited in the level of transcription by lowering the expression of integrin on fibroblasts in scleroderma.
