Effects of propufol on nitric oxide synthase activity and nitric oxide output of rat brain in vivo
- VernacularTitle:异丙酚对大鼠不同脑区NOS活性和NO产量的影响
- Author:
Xingguo HU
;
Jun WANG
;
Yinming ZENG
- Publication Type:Journal Article
- Keywords:
Propofol Brain Nitric oxide Ligases
- From:
Chinese Journal of Anesthesiology
1996;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of propofol on nitric oxide synthase (NOS) activity and nitric oxide (NO)output of rat brain. Method: Sixteen SD rats were divided randomly into two groups. The animals were administered introperitoneally(ip) normal saline 10 ml?kg~(-1)(control group)or propofol 100mg?kg~(-1)(propofolgroup),respectively. These rats were decapitated immediately after having disappeared righting reflex. After rapid removal of cerebellum, brain stem,hippocampus and cerebral cortex,tissues were homogenized and centrifuged. NOS activity and NO output were assayed with spectrophotometric analysis. Result: In propofol group,NOS activity was significantly inhibited, NO outpul was significantly reduced in cerebellum, brain stem,hippoeampus and cerebral cortex as compared with those of control group(P
