The changes of IL-6 gene expression in acute repetitive hypoxia
- VernacularTitle:急性重复性缺氧时原代培养脑细胞中白介素-6基因表达
- Author:
Juan KONG
;
Weimin CHENG
;
Jianjun CUI
- Publication Type:Journal Article
- Keywords:
Interlaukin-6;
Gene expression;
Cell;
cultured;
Brain;
Acute repetitive hypoxia
- From:
Chinese Journal of Anesthesiology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study was to observe the changes of IL-6 gene expression in acute repetitive hypoxia. Method: When the ratio of living cells was more than 95%, nerve cells cultured were passed through with mixed gases of 95% N_2+5%CO_2 for 3 min followed by another gases mixture of 95% O2+5% CO_2 for 10 min. After repeated the experiment as above, the alteration in the expression of IL-6 gene was measured using the RT-PCR method. The prod ucts of PCR were analyzed with computer Gel imaging and image analysis instrument. Result: After the first hypoxia IL-6 gene expression enhanced, and after the second hypoxia, it was still stronger than baseline although it declined slightly. There was no significant RNA disintegration. Conclusion:The IL-6 involves in the cellular defense in hypoxia adaptation.
