Preparation of Desmoglein 1,3 Type Probes Using PCR of Incorporation of Dig-dUTP Method
- VernacularTitle:聚合酶链反应Dig-dUTP掺入法制备桥粒芯蛋白1、3型探针
- Author:
Gangwen HAN
;
Lijun ZHOU
;
Shengqing MA
- Publication Type:Journal Article
- Keywords:
Desmoglein;
Probes
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study we successfully prepared desmoglein 1 and desmoglein 3 DNA probes by incorporation of Dig-dUTP during PCR amplification. Eleetrophoresis analysis showed that Dig labelled probes moved slower on the gel than PCR products not incorporated with Dig due to the enlargement of probe fragment. The results of Dot-blot showed that the prepared probes can be used for RNA analysis, and both probes have high specificity, The results provided basis for the quantitative and qualitative study of desmoglein gene expression in tissues or cells by means of in situ hybridization and Northern hybridization etc.
