Effect of interleukin-13 on the growth of human erythroleukemia cell line
- VernacularTitle:白细胞介素—13对人红白细胞白血病细胞株的作用“英文”
- Author:
Xiaoyu SHI
- Publication Type:Journal Article
- Keywords:
Interleukin- 13 ? Megakaryocytes ? Glycoproteins
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
In this study we investigated the effect of recombinant human interleukin-13 (IL-13) on the growth of human erythroleukemia cell line(HEL)-a human megakaryobiastic leukemia cell line. HEL cells were cultured in the presence or absence of IL-13. After incubation for 4 days, the cells were first counted and then stained with a direct immunofiuorescence method for flow cytometric analysis of the expression of glycoprotein(GP) Ⅱ b/Ⅲ a. In order to examine the effect of IL-13 on the proliferation of HEL cells, the immunocyte assay was used for the detection of 5-bromo-2'-deoxyurid:ine (5-Brdu) incorporated into the DNA of HEL cells after they had been cultured for 3 days. We found that the expression of GP Ⅱ b/Ⅲ a on IL-13 treated HEL cells was increased to a significant degree (P
