Detection of HPV DNA in Condylomata Acuminata by Direct in Situ PCR
- VernacularTitle:直接原位聚合酶链反应检测尖锐湿疣中的人乳头瘤病毒
- Author:
Hui LI
;
Xuejun ZHU
- Publication Type:Journal Article
- Keywords:
Condylomata acuminata Polymerase chain reaction Papillomaviruses
- From:
Chinese Journal of Dermatology
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study is to ascertain the significance of diagnosis of human papillomavirus (HPV) infection and the histological pattern of distriution of HPV in condylomata acuminata(CA) by direct in situ PCR (ISPCR) technique. of the 25 patients with BA, all 10 cases with typical histopathological features of CA gave a positive reaction in tile upper layers of the epithelium. HPV was also detected in the granular ladyer and whole prickle cell layer of 11 cases of CA with no typical koilocytes. In the direct ISPCR oligonucleotides labelled with bio tin were incorporated in situ during amplification, then immunohistochemical method was used for detection. In this technique the viral existence in paraffin sections can be directly compared with the histologic changes, and can help pathologist to analyze the possibility that HPV is present in the suspected lesions.
