Amplification of 160 000 Bullous Pemphigoid Antigen cDNA Fragment from Human Keratinocyte Library
- VernacularTitle:从人角阮细胞cDNA文库中扩增160000类天疱疮抗原片段
- Author:
Guanqun LI
;
Xuejun ZHU
- Publication Type:Journal Article
- Keywords:
Bullous pemphigoid;
Polymerase chain reaction
- From:
Chinese Journal of Dermatology
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Bullous pemphigoid antibodies can recognize two autoantigens-BPAG1 (220 000-240 000 ) and BPAG2 (160 000 -180 000). With two specific primers, we successfully amplified a 588bp cDNA fragment of 160 000 BP antigen from human keratinocyte cDNA library in this paper. The result confirmed that lip antigens were synthesized by keratinocyte at a molecular level. This cDNA fragment could not be amplified with epidermal DNA Templets. We suggest that BP antigen gene may be separated by some introns. We will prepare cDNA probes and recombinant antigens using PCR product in order to elicit autoimmunity mechanism of BP.
