AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF pYNZ22 LOCUS IN 120 CHINESES AND ITS APPLIFICATION TO FORENSIC SCIENCE PRACTICE
- VernacularTitle:pYNZ22位点扩增片段长度多态性法医应用的研究
- Author:
Lan HU
;
Jintang NI
;
Song CHEN
;
Anquan JI
;
Jian YE
;
Boling LI
- Publication Type:Journal Article
- Keywords:
pYNZ22 Locus;
Amp-FLP;
Silver Stain
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A region of the D17S30(pYNZ22) locus was amplified in DNA from 120 unrelated chinese individuals was carried out. The amplified products were analysed by mini-polyacrylamide gel electrophoresis followed by silver stain. 11 alleles, ranging from 170bp to 870bp in size and from 0. 4 % to 30. 4 % in frequency, was detected. The heterozygosity was 73%, Dp value was 0.938. The family study showed that the Amp-FLP in pYNZ22 locus is inherited according to the Mendelian law. The correct genotyping results can be obtained from very little amount of biological material, such as mixed stains, blood stains, single hair and saliva.