ISOLATION AND PURIFICATION OF HUMAN FIBRONECTIN,PREPARATION OF ANTISERA AND ITS PRACTICAL USE
- VernacularTitle:人血浆Fn的纯化、抗体制备及法医学应用的研究
- Author:
Fan LI
;
Qiyin ZHANG
;
Yiju CHEN
;
Ziqin ZHAO
;
Wenqing WU
;
Yunju GU
- Publication Type:Journal Article
- Keywords:
Affinity chromatography;
Anti-fibronectin serum;
Injury
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The fibronectin was purified from human plasma by Gelatin-Sepharose 4B affinity chromatography. The rabbits were immunized with the fibronectin. the raw anti-fibronectin sera were absorbed bythe solid phase of the fibronectin free human serum. Then the specific anti-fibronectin sera were obtained. The fibronectins deposited on human bullet wounds, incised wounds as well as on blunt forceinJuries were demonst.ated by PAP immunohistochemical study using diluted anti-fibronectin serum.The results were quite sati factory.
