Effects of Aprotinin on Acute Lung Injury by Endotoxin in Rabbits.
10.4097/kjae.2001.41.5.608
- Author:
Ji Yeon SIM
1
;
In Cheol CHOI
;
Su Kyoung CHOI
;
In Hea CHO
;
Hong KO
Author Information
1. Department of Anesthesiology, College of Medicine, University of Ulsan, Korea. jysim@amc.seoul.Kr
- Publication Type:Original Article
- Keywords:
Bacteria: endotoxin;
Lung: acute lung injury;
Pharmacology: aprotinin
- MeSH:
Acute Lung Injury*;
Animals;
Aprotinin*;
Blood Cell Count;
Cardiopulmonary Bypass;
Edema;
Fibrinogen;
Hemodynamics;
Ketamine;
Lung;
Lung Injury;
Mortality;
Oxygen;
Partial Pressure;
Partial Thromboplastin Time;
Permeability;
Peroxidase;
Prothrombin Time;
Rabbits*;
Respiratory Distress Syndrome, Adult;
Serine Proteases;
Thoracic Surgery;
Vascular Resistance;
Vecuronium Bromide;
Xylazine
- From:Korean Journal of Anesthesiology
2001;41(5):608-619
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Aprotinin, a serine protease inhibitor, has an anti-inflammatory and hemostatic effect and has been used to reduce perioperative blood loss and lung injury after cardiopulmonary bypass in cardiac surgery. Acute respiratory distress syndrome (ARDS), which results in clinical manifestations due to non-cardiogenic permeability edema is a fatal condition associated with a mortality rate of 50 to 80%. The purpose of this study was to evaluate the effects of aprotinin on acute lung injury induced by bacterial endotoxin in rabbits. METHODS: Nineteen rabbits were anesthetized with intravenous xylazine, Ketamine and vecuronium and ventilated with a Harvard apparatus maintaining normocapnea. In 7 rabbits, 2 mg/Kg of lipopolysaccharide from E. coli was infused intravenously for 30 min (Toxin group) and in another 7 rabbits aprotonin loading with 200,000 KIU/Kg followed by a continuous infusion of 50,000 KIU/Kg/hr was performed 30 min before the endotoxin infusion throughout the experiment (Aprotinin group). At 1, 2, 3, and 4 hours after endotoxin infusion, arterial blood gas, blood cell count, prothrombin time, activated partial thromboplastin time, fibrinogen, and hemodynamic profiles were checKed. At four hours, the animals were dissected at which time the lungs were divided into three regions for wet/dry weight ratio (WW/DW), myeloperoxidase activity and microscopic examination. RESULTS: In the Aprotinin group, pulmonary vascular resistance, arterial oxygen partial pressure and coagulation function were well preserved compared with the Toxin group. Furthermore, lung WW/DW, myeloperoxidase activity, and inflammatory responses also increased less in the Aprotinin group. CONCLUSIONS: The results of the current data showed that aprotinin has prophylactic effects against acute lung injury and coagulation impairment induced by bacterial endotoxin in rabbits.
