RFLPs ANALYSIS OF DIGOXIGENIN-LABELLED pAW101 PROBE AND ITS PRACTICAL USES
- VernacularTitle:用地高辛配基标记的pAW101探针杂交分析DNA多态性及其应用
- Author:
Chao LIU
;
Chaoquan LUO
;
Yinghao YANG
;
Xinyao WU
;
Jianyang LUO
- Publication Type:Journal Article
- Keywords:
Restrtion fragment length polymorphisms (RFLPs) Digoxigenin Paternity determination
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A new method to revel RFLPs is presented. The human genomic DNAwas purified by saturatedNaCl solution and the pAW101 probe labelled with digoxigenin-dUTP. The relationships of RFLPsand genetic patterns of PGM1 (phosphoglucomutase),EsD (esterase D),GLO1 (glyoxalase)and ACP(acid phosphatase ) between the fillal generation and parental generation were detected in 15 families(among them 11 cases were aborted fetuses). The probability of paternity (w)was caculated accor-ding to Essen - Moller's formula, each w vlua was over 99. 73 %, reached the standard of incladingpaternity. An effective,rapid, and non-toxic RFLPs technique was established, which is easy to man-age in common lab oratories.
