PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY AGAINST HUMAN ALLOTYPE G2M(23)
- VernacularTitle:抗人同种异型G2m(23)单克隆抗体研制及特异性鉴定
- Author:
Yingsong KUAI
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody Allotype G2m(23) ELISA
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
One hybridoma cell line (2G8) secreting monoclonal antibody against humn allotype G2m (23 )was produced by fusion of myeloma cell (SP2/0) and spleen cells from BALB/c mice immunized withlgG2 [G2m (23 + )], isolated and purified with FPLC protein A column from human plasma. Themonospecificity of McAb against G2m (23 ) antigen was testified by ELISA-inhibition, Sandwich -ELISA and Dot-ELISA test.
