PREPARATION OF RNA PROBE OF MINISATELLITE REGION IN HUMAN DNA AND ITS PRACTICAL USE(Ⅰ)-CONSTRUCTION OF SUB-CLONE OF pGEM-4Z-MYO
- VernacularTitle:人类DNA小卫星区域RNA探针制备及应用的研究(Ⅰ)──pGEM-4Z-MYO亚克隆的构建
- Author:
Yuxin LI
- Publication Type:Journal Article
- Keywords:
Minisatelite DNA DNA recombinant techniques Sub-clone
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
By means of DNA recombinant techniques, the fragment of MYO minisatellite DNA probe 0b-tained from plasmid pUC19 - MYO was inserted into plamsid pGEM - 4Z containing RNA poly-merase promotor, thus a sub-clone pGEM- 4Z-MYO was constructed then. It provided the RNAprobe for the DNA fingerprinting and laid a foundation for raising the sensitivity of detecting the poly-morphism of the minisatel1ite DNA.
