DETECTION OF THE IMMUNE COMPLEX IN KIDNEYS OF ANAPHYLACTIC SHOCK ANIMALS
- VernacularTitle:过敏性休克死亡动物肾脏免疫复合物的实验研究
- Author:
Zhijin ZUO
;
Jiazhen ZHU
- Publication Type:Journal Article
- Keywords:
Immunocomplex;
IgE;
Immunohistochemical method;
Anaphylactic;
shock
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper reports the immunohistochemical changes in kidneys of anaphylactic shock animals byAvidin-biotin-per-oxidase Complex Technique (ABC), Peroxidase - anti - perox idase Complex Technique(PAP)and Lablled Avidin-biotin Technique (LAB). The immediate naphylaxis was always accompanied by The immunocomplex formation in kidney. the immunocomplex adhered to the mesangial cells,the endothelial cells of capillaries and small arteries,and also to the adventitia of blnd vessels. In addition to allegens,such as penicillin and exogenous serum, IgE and IgG were observed in theimmuno-complex. This finding suggested that the IgE in the immunocomplex might be characteristicof type I anaphylaxis and could be distingushed from other kind of immunocomtplexs. Animals keptedat the room temperature (22 - 25℃)for 6 or 12 hours, or in refrigerator (4 ℃ )for 48 hours would notinfluence the positive reactions. This method seems feasible for practical use in the casework of anaphylactic shock.
