Comparative Study for DNA Extraction Methods from femurs in case of naming unidentified bodies.
- Author:
Eung Soo KIM
1
;
Dong Sub LEE
;
Jin Hee KIM
;
Moon Hee PARK
;
Hee Jung AHN
;
Young Joo KIM
;
Dae Yeol KIM
;
Sung Jin CHO
;
Byung Ha CHOI
;
Young Shik CHOI
Author Information
1. Forensic Medicine Division, Eastern District , National Institute of Scientific Investigation, Wonju, Korea. pooh@nisi.go.kr
- Publication Type:Comparative Study ; Original Article
- Keywords:
DNA extraction;
Ethanol down method;
Microcon YM-100;
DNA IQ(TM) system
- MeSH:
Disasters;
DNA Fingerprinting;
DNA*;
Ethanol;
Femur*
- From:Korean Journal of Legal Medicine
2007;31(1):41-44
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In historical cases, missing persons' identification, mass disasters, and ancient DNA investigations, bone samples are often the only and always the best biological materials available for DNA typing. There are many protocols for the extraction of DNA from bone samples. In our experiment, we tested three DNA extraction protocols: Ethanol down method, Microcon(R) YM-100 and DNA IQ(TM) system. We have found that ethanol down method is not suitable to extract DNA from bone samples and Microcon(R) YM-100 is the best among these three mothods. DNA IQ(TM) System did not show better result but needed less time and labor than Microcon(R) YM-100. So, we expect using DNA IQ(TM) System is useful in case of bone samples which are not severely contaminated .
