Preliminary Studies on Characteristics of Two Monoclonal Antibodies of Anti-Mouse Pancreatic Cancer
- VernacularTitle:两株抗小鼠胰腺癌McAb特性的初步研究
- Author:
Yun WU
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Mouse;
McAb
- From:
Journal of Kunming Medical University
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Two monoclonal antibodies of anti-mouse pancreatic cancer, 2B_6and 2B~(10), were purified by the use of salt fractionation-exchange chromatography(DEAE-52) or affinity chromatography from culture supernatants and mice ascites of the hybridomas. After assayed by the use of SDS-PAGE and labeled with FITC, they were used to analyse related antigen site of 35 tissue samples of several human tumor by means of direct-fluorescence method. There was an intense positive reaction between 2B_(10)and 4 samples of gastric cancer. 2B_6or 2B_(10)and 3 tissur samples of ovary cancer and 1 clonal cell of ovary cancer also had intense positive reaction. These results showed that there exist common tumor antigen in both human and mice.
