1-Butanol extracted low molecular weight antigen of cervical cancer cell surface
- VernacularTitle:正丁醇提取的低分子量宫颈癌细胞表面抗原
- Author:
San WU
;
Suxun WU
- Publication Type:Journal Article
- Keywords:
Antigens,neoplasm;
Cervix neoplasms;
Alconols,butyl;
Antibodies,monoclonal;
Mice
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Cervical cancer (U_(14)) cell surface antigen induced by methylcholanthrene was extracted by 1-butanol. The U_14 cells were incubated in 2. 5% 1-butanol, the resul-ted supernatants were referred to as CBE (crude butanol extract). Protein concentration ofCBE determined by the Lowry assay was 147?g/ml. CBE was characterized by SDS-PAGE, which showed eight bands on gel. Their molecular weight range was found to be18-70 kD. CBE was tested by indirect ELISA, which showed that CBE had positive reac-tion with both monoclonal (AU_(14-1)) and polyclonal antibodies. These results indicatedthat CBE contained low molecular cervical cancer cell surface antigen and the antigenicdeterminant which binds to AU_(14-1) specifically.
