Determination of Propafenone Concentration in Human Plasma by High-performance Liquid Chromatography
- VernacularTitle:高效液相色谱法测定普罗帕酮血浆浓度
- Author:
Zhengzhong WU
;
Rongsheng TONG
- Publication Type:Journal Article
- Keywords:
propafenone HPLC
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and simple HPLC method for determination of propafenone concentration in human plasma is described. Dyclonine hydrochloride was used as an internal standard. YWG-C_(18) column(10?m)was used with a mobile phase of methanol-acetonitrile-0.05mol/L acetate buffer(pH 4.02)-diethylamine(45.5:19.5:35). Detection was performed by ultraviolet absorption at 254nm. The detection limit of propafenone waw 5ng (S/W=3)or 25ng/ml in plasma. Assay linearity was shown in a range of 50～500ng/ml with a regression coefficient of 0.9993. The recovery(n=4)was 100.5％,the CV of within-day and day-to-day being less than 3％. No interference was found from endogenous compounds or commonly used drugs. So it can be applied to pharmacokinetic studies and therapeutic monitoring.
