A time—related study about morphometry in myocardial ultrastructure and Ca distribution in ischemia and reperfusion damage and the protective effect of Danshen for the heart
- VernacularTitle:缺血/再灌注时心肌细胞线粒体钙超载及保护的超微结构“定量”观察
- Author:
Fang LI
;
Xiaoming WANG
- Publication Type:Journal Article
- Keywords:
Myocardium;
Perfusion;
Calcium;
Mitochondria;
Heart
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Calcium overload has been discovered in the ischemia and reperfusion injury of the heart recently, but there were less reports about changes in myocardial Ca distribution under this same condition by electronmicroscopy. Using the potassium-pyroantimonate method and X-ray microanalysis, we studied cytochemical calcium localization in the rabbit's myocardium at various time periods of ischmia with or without subsequent reperfusion. At the same time, we had the morphometric estimation of Ca granule volume density in the mitochondria by TAS(texture analysis system) and evaluated Danshen's protection for ischemia and-reperfusion injury. The results showed that mitochondrial Ca granule volume density increased significantly with the development of ischmia and post-ischemic reperfusion, especially during reperfusion following 40min of ischemia, however following 60min of ischemia, Ca granule volume density decreased than that observed following 40 min of ischemia. This meant that the limit time point for reversible and irreversible injury could be in the range of 40～60min of ischemia. Danshen pretreatment to the rabbits reduced Ca granule volume density significantly. This demostruted that Danshen acted as a calcium antagonist and contributed an outstanding protection to the myocardial calcium overload.
