The effects of several tumor promoters on gap junction intercellular communication in NIH/3T3 cells
- VernacularTitle:几种促癌剂对NIH/3T3细胞经缝隙连结细胞间通信的影响
- Author:
Xianlong GAO
;
Yingnian YU
- Publication Type:Journal Article
- Keywords:
Communication;
Carcinogens;
Protein kinases
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of tumor promoter TPA, MZR and PB on gap junction intercellular communication in NIH/3T3 cells were studied using the scrape-loading/dye transfer technique. All of these 3 agents were shown to cause a dose-dependent inhibition of the intercellular communication. The blockage of intercellular communication induced by TPA and MZR was inhibited in the presence of the protein kinase C inhibitor, staurosporine or palmitoyl carnitine, but not inhibited in the casexinduced by PB. The results suggest that the serape-loading/dye transfer technique may be used as a rapid screening assay to detect the tumor promoters and protein kinase C may be involved in the genesis of intercellular communication blockage induced by TPA ane MZR, but not by PB.
